Tài liệu, ebook Khoa Học Tự Nhiên

Acetyl-CoA Carboxylase acetyl-CoA + CO2 + ATP  malonyl-CoA + ADP + Pi 1St committed step in fatty acid biosynthesis In presence of citrate activated In presence of fatty acyl-CoA inactivated

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Ping-Pong Reactions In Ping-Pong rxns first product released before second substrate binds When E binds A, E changes to F When F binds B, F changes back to E

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study of reaction rate determines number of steps involved determines mechanism of reaction identifies “rate-limiting” step

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Sickle cell anemia – E6 to V6 Causes V6 to bind to hydrophobic pocket in deoxy-Hb Polymerizes to form long filaments Cause sickling of cells Sickle cell trait offers advantage against malaria Fragile sickle cells can not support parasite

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Stabilize native structure Formed after native conformation achieved Abundant in secreted proteins but not in intracellular proteins Protein disulfide isomerase catalyzes reduction of incorrect disulfide linkages

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Loops Loops usually contain hydrophillic residues. Found on surfaces of proteins Connect alpha-helices and beta-sheets Turns Loops with < 5 AA’s are called turns Beta-turns are common

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Trypsin Met-Ala-Arg Phe-Ala-Glu-Gln-Asp Gly-Glu-Tyr-Met-Cys-Lys Chymotrysin Met-Ala-Arg- Gly-Glu-Tyr Met-Cys-Lys –Phe Ala-Glu-Gln-Asp CNBr Met Ala-Arg-Gly-Glu-Tyr-Met Cys-Lys-Phe-Ala-Glu-Gln-Asp

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Draw the decapeptide at pH 1, 7, and 12. (pay attention to the form the N- and C- terminal and each R-group takes on at each pH) Calculate the overall charge at each pH. Write out the one letter code for the decapeptide

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Buffers are aqueous systems that resist changes in pH when small amounts of a strong acid or base are added. A buffered system consist of a weak acid and its conjugate base. The most effective buffering occurs at the region of minimum slope on a titration curve (i.e. around the pKa). Buffers are effective at pHs that are within +/-1 pH unit of...

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The National Center for Biological Information (NCBI) website contains a vast store of biological information, including genomic and proteomic data Start with a sequence and discover gene to which it belongs, then compare that sequence with that of similar genes Query the database with a topic for information View structures of protein in 3D by...

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