Free Energy Change in Glycolysis Control Points in Glycolysis Effect of ATP on PFK-1 Activity
15 trang | Chia sẻ: nguyenlam99 | Ngày: 11/01/2019 | Lượt xem: 1012 | Lượt tải: 0
Substrate level phosphorylation generates second ATP Large, negative G - regulation! Allosterically activated by AMP, F-1,6-bisP Allosterically inhibited by ATP and acetyl-CoA
24 trang | Chia sẻ: nguyenlam99 | Ngày: 11/01/2019 | Lượt xem: 1066 | Lượt tải: 0
Enzyme Regulation of Flux Common mechanisms Feedforward activation
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Common unsaturated fatty acids Unusual fatty acids can function analogously to unsaturated Enzymes used to Dissect Phospholipid Structure
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Structure of the ABO blood group carbohydrates,R represents the linkage to protein in the secreted forms, sphingolipid in the cell-surface bound form open square = GlcNAc, open diamond = galactose, filled square = fucose, filled diamond = GalNAc, filled diamond = sialic acid (NANA)
19 trang | Chia sẻ: nguyenlam99 | Ngày: 11/01/2019 | Lượt xem: 1043 | Lượt tải: 0
Glucose Fructose Ribulose Glyceraldehyde Dihydroxyacetone
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Protein coenzyme Heme containing proteins Fe3+ can undergo reversible one electron reduction Impt in redox rxns Classified based on the basis of their visible absorbance spectra
16 trang | Chia sẻ: nguyenlam99 | Ngày: 11/01/2019 | Lượt xem: 1071 | Lượt tải: 0
Thiamin is the first Vitamin discovered (Vital amine = Vitamin) Deficiencies lead to disease called Beriberi (neurological disorders, heart problems, anorexia) Beriberi prevealent in undeveloped countries where polished grains make up the majority of the diet. Associated with alcohol related disorders (Wernickes-Korskofff syndrome – memory loss,...
22 trang | Chia sẻ: nguyenlam99 | Ngày: 11/01/2019 | Lượt xem: 1034 | Lượt tải: 0
Trypsin, chymotrypsin, elastase, thrombin, subtilisin, plasmin, TPA All involve a serine in catalysis - thus the name Ser is part of a "catalytic triad" of Ser, His, Asp (show over head) Serine proteases are homologous, but locations of the three crucial residues differ somewhat Substrate specificity determined by binding pocket
35 trang | Chia sẻ: nguyenlam99 | Ngày: 11/01/2019 | Lượt xem: 1060 | Lượt tải: 0
Some enzyme catalyzed rxns show more complex behavior E + S<->ES<->EZ<->EP<-> E + P With M-M can look only at rate limiting step Often more than one substrate E+S1<->ES1+S2<->ES1S2<->EP1P2<-> EP2+P1<-> E+P2 Must optimize one substrate then calculate kinetic parameters for the other Assumes k-2 = 0 Assume steady state conditions
22 trang | Chia sẻ: nguyenlam99 | Ngày: 11/01/2019 | Lượt xem: 1051 | Lượt tải: 0