Screen master Lihd113 - User manual v. 3.1 06/04 english

SCREEN MASTER LIHD113 USER MANUAL V. 3.1 06/04 ENGLISH HOSPITEX DIAGNOSTICS Via Provinciale Lucchese 145 - 50019 Sesto Fiorentino (Florence) Italy Tel. +39 055 374083 Fax + 39 055 374084 e-mail : hospitex@hospitex.it web : I N D E X page DESCRIPTION OF THE ANALYSER................................... 2 Technical specifications ... 2 MEASURMENT PRINCIPLES............................................. 3 INSTALLATION ................ 4 Packing contents 4 Keyboard ...... 5 Rear panel setting ....... 6 Location of the instrument .... 6 Power supply connection .. 6 Caution .... 7 Reset and switching off.................................................................... 7 Measurement block .... 8 Manual reading mode 8 Tube setting .... 9 Printer settings .... 10 Incubator ...... 10 INSTRUMENT UTILISATION . 11 Introduction . 11 Programming methods ... 12 New methods 13 Modify method . 24 Erase method . 25 Working on methods 26 Zero-setting .. 28 Sample blank zero setting 28 Production of standards ... 30 Sample measuring .37 5.4 ABS mode ... 41 5.4.1 ABS Mode Selection Screen . 41 5.4.2 ABS Mode Measurement Screen 42 5.5 Washing ... 42 5.6 Print Menu ...43 5.6.1 Printing Options Screen . 43 5.7 Using manual cuvette ...... .45 MAINTENANCE . 46 Cleaning procedures ......................................................................... 46 First installation .................................................... 46 Everyday cleaning .................................................... 46 Special cleaning .....46 6.2 Further advice................................................................................47 6.3 Changing lamp procedure 47 Thank you for choosing Hospitex Diagnostics SCREEN MASTER equipment. This instrument was designed to have powerful features in a compact unit for biochemical investigations, enzymes, immunoturbidimetrics, and drug tests. These characteristics make the Screen Master a perfect laboratory instrument. If you have any questions or trouble operating the equipment please contact support@hospitex.it DESCRIPTION OF THE ANALYSER The Screen Master technical features are: - Multilingual capability. More than 200 programmable tests. Designed for easy use due to step-by-step procedures. - Built-in eight position incubation block. - Integrated pump and flow cell. - Internal pre-adjusted peltier element at 25°C, 30°C, 37°C. It performs: - End Point - Kinetic - Fixed Time Multistandard assays Results are shown on graphic display and printed on paper by built-in printer. Technical specifications Light source Halogen lamp –12V, 20 W Wavelength 340nm-700nm. Wavelength selection Automatic via 8-position filter wheel; 6 standard interference filters: 340nm, 405nm, 505nm, 546nm, 578nm, 630nm. Two free positions for optional filters. Photometric Range: 0-2.5 O.D. Flow cell system 32mL flow cell with 10mm light path, interchangeable with disposable macro, semi-micro, or special optical glass cuvettes. Reading time 1s-300s. Incubation time 10s-600s. Temperature control Peltier elements, 25°C, 30°C and 37°C. Reaction volume 500 mL per test. Display Graphic, 21 characters per 8 lines. Printer Graphic, 24 characters per line. Delay time 5 sec. Dimensions Length 33cm x width 34cm x height 18cm. Weight Kg 8.5 Power supply 230 AC 50-60 Hz. MEASUREMENT PRINCIPLES The concentration of a solute that absorbs light at a determinate wavelength can be determined with a spectrophotometer. The fraction of light absorbed is proportional to the concentration of the absorbing solute (Beer’s law): (1) I / I0 = e-kc Where: I0= Incident light intensity before going through to sample. I= Transmitted light intensity through to sample. K= Constant that depends upon the length of light path, nature of the substance and the light wavelength used. C= Substance concentration in solution. Equation (1) can be changed into: log( I0/I) = kc The quantity log(I0/I) is called Optical Density (OD) or Absorbance (ABS) and is proportional to concentration. A spectrophotometer measures the intensity of a known wavelength (measured in nanometers, nm) of light passed through the sample: OD = log( I0/I) By using specific reagents in the solution for the substance that must be measured, it is possible to estimate the constant ( k) for a fixed wavelength, in this way, transmitted light intensity becomes a concentration function. The instrument has a reading point kept at 25-30-37°C temperature and in order to perform a measurement the following will be necessary: Analysis with flow cell using a peristaltic pump. Keeping manual cuvette at 37°C temperature using external incubator. The measure chain could be represented by the following scheme: receiver A / D sample Selective filter and focal system Amplification Analogic section Digital section I0 I White light INSTALLATION Packing contents Inside the package is a starting kit composed of the items listed below which explains how to correctly install the instrument. If any of the following items is missing or damaged, please contact Hospitex for help. - Power supply cable. - Two fuses. - Waste bottle and silicone tube. - One hundred macro cuvette. - Two paper rolls. - Instrument cover lining. - Cylinder for manual readings Two cuvette adapters: Manual Cuvette ADAPTER Flow Cell ADAPTER (500 µL reagent Min. quantity for flow-cell measurement) (500 µL reagent Min. quantity into manual cuvette) 3.2 Keyboard Correct use of the keyboard is very important for performing analyses and for obtaining fast and accurate operations. KEY FUNCTIONS 0 - To insert ‘0’ and to set parameters to zero in ABS mode. from 1 to 3 - To insert numbers and to choose between the options. from 4 to 9 - To insert numbers. ? - Print of Kinetic and Fixed Time Dynamics in the ‘Sample Measurement Screen’. Right and left arrows - To move on main screen. - To move on values. - To move between several menu options. Up and Down arrows - To move on main screen. - To move between several menu options. - To scroll up and down the method list in ‘EXE’, ‘PRG’ and ‘PRT’. OK - To confirm your choices. Del - To exit from the current Menu (quick press) - Paper feed (press continuously for more than 3 seconds) (.) -To wash Flow-Cell ( Activate peristaltic pump) Rear panel setting Located in the rear panel (from left to right as shown in the picture) you will see: Fan for internal cooling ( a duplicate is located under the instrument). The power supply switch. OUTLET POLYSNAP FAN The waste outlet. Before switching on the instrument, remember to connect the plastic outlet connector (red) to a waste tank by means of a silicone tube. Location of the instrument The instrument should be located in a clean environment, placed on a stable surface, and away from direct sunlight which could affect the operating temperature and the quantity of light measured by the instrument. The following points should be taken into consideration. - Ensure that it is on a level surface. - Avoid positions subject to jerks or vibrations. - Make sure that the instrument is not placed close to air conditioning or heat sources. - For the long life of the instrument these temperature conditions should be followed. 5°C - 50°C for instrument storage. 15°C - 30°C for instrument use. Power supply connection Please check the setting of the power supply switch according to your country’s electrical network. Connect the power plug to a grounded AC wall outlet, preferably one that is not shared with other electric appliances and with low fluctuation of line voltage compared to the standard voltage specified (10-15%). -Keep the instrument away from other appliances that generate high frequency electrical noises (e.g. radiological instruments). - Before connecting the power cord, check that the AC power supply corresponds to the value that is stated on the instrument’s label. Labels: For countries with 110 AC voltage supply: For countries with 230 AC voltage supply: HOSPITEX DIAGNOSTICS Model : SCREEN MASTER Code : LIHD113 S/N : XXXXXXX AC 110V 50/60 Hz 75W Fuse 4A Made in Italy HOSPITEX DIAGNOSTICS Model : SCREEN MASTER Code : LIHD113 S/N : XXXXXXX AC 230V 50/60 Hz 75W Fuse 2A Made in Italy 4.6 Caution Do not connect the instrument to a power supply different from the value indicated on the label. - Before connecting the power and finishing the installation section, make sure that the instrument is turned off (check the polysnap located on the rear part of the instrument). - Make sure that your AC main line has an efficient ground line. A bad ground line connection may compromise analysis results and damage the instrument. - After turning on the instrument, Pay attention not to spill liquids or micro solid substances on the surface around the instrument. - Keep the instrument away from young children. If the above procedures are carefully followed, it will be possible to TURN ON the instrument by using the switch located on the polysnap block. Reset and switching off To reset the instrument: turn off the switch. wait for 5 seconds. turn on the switch. Remember that the instrument must only be reset if the software does not work. Measurement block Open the measurement block cover, it is found on the top left part of the instrument. The parts are as follows: a peristaltic pump that allows the solution to enter the instrument for measurement. an incubator well which keeps the flow-cell or manual cuvette at the temperature of approximately 37°C. an inlet tube. Instruction for a correct aspiration: Prepare disposable cuvettes with correct amount of reagent and sample volume according to the method. Set aspiration tip inside cuvette, be sure that aspiration tip is laying in one corner of the cuvette (see figure A) Then press PUSH BUTTON, the sample will be aspired automatically. Figure A Aspiration tip Cuvette Manual reading mode To perform a measurement manually with a disposable cuvette, see the following instruction: Switch off the instrument Extract the flow-cell and its adapter from the reading well and leave it free inside the measurement block Insert manual cuvette adapter into reading well Now it is possible to read manual cuvette, insert cuvette into reading well and cover it with the black cylinder (from equipment kit) Close measurement block door and press PUSH BUTTON to read the sample Repeat same operation for the following samples Tube setting Make sure that the transparent tube (the one that connects the flow-cell with the internal hydraulic circuit) is not twisted or squashed. Place the red tube around the peristaltic pump and make sure that the white plastic connections are set as shown in the figure below: RED TUBE INLET TUBE PUMP CUVETTE POSITION Under the measurement block there is a lever (the PUSH button). Press the lever for no more than half a second to pump samples into the flow cell. PUSH button INLET PIPE MEASUREMENT BLOCK DOOR Printer settings To insert the paper roll, proceed as follows: - Switch on the instrument. - Remove the printer cover and locate the green lever. - Raise the little green lever located on the right side of the printer. - Take a new paper roll and pass the edge of the paper under the black rubber cylinder. The cylinder will start its rotation so that the paper will set itself automatically. Pull the lever down again, pass the edge of the paper through the aperture and place the printer cover in its proper position. GREEN LEVER Incubator The incubator temperature is brought up to 37 °C by the software and it will remain constant until the instrument is turned off. NOTE: It is very important to heat the macro cuvettes to the proper temperature for obtaining the most accurate analysis results. Built-in incubation block. 4. INSTRUMENT UTILISATION Swicth on the instrument. PLEASE PAY ATTENTION: DO NOT TOUCH THE KEYBOARD UNTIL THE CHESS-BOARD APPEARS Introduction The first screen will show : USER: Operator code (choose numbers from 00 to 99) DATE: Current working date USER : 00 DATE 00 / 00 / 00 Insert USER and DATE by using keyboard numbers, to correct move on number using right and left arrows keys: . Then press OK key to proceed to main menu screen. The operator code and date will be displayed at the beginning of each method analysis. Main menu screen: XX.X °C EXE PRG ABS PRT The main menu is composed of four options which can be selected by scrolling over the icons. Every option is linked to a function of the instrument: EXE. Execution of the methods saved in memory. ABS. ABS test on sample with programmable volume and filter with zero option. PRG. Writing and saving, viewing and erasing methods in memory. PRT. To print list and methods on thermal paper. The main menu, displayed on the top of the main screen, allows to check the incubator temperature. Selection keys Use the arrow keys , to move to different options over the screen, and then press OK to confirm. 5.2 Programming methods Choose PRG from the main screen then press OK and it will appear: Intro Screen PROGRAM. 1 NEW 2 MODIFY 3 ERASURE There are three options: NEW MODIFY ERASURE Selection keys There are two ways to select: 1) Use the up & down arrow keys to move to the different options over the screen, then press OK to confirm. 2) Use the number keys to make a selection by the inserting row index, for example insert (1) to have a new method. 3)Use DEL to return to the MAIN SCREEN. 5.2.1 New methods 5.2.1.1 Name screen NEW 001 NAME: [ xxxxxxxxxxxxxxxxx] Insert the name first. The name will be saved in the memory in this format: ORDER NUMBER (given by micro controller) + METHOD NAME (inserted by user). Selection keys: Use the up & down arrow keys to select character (A..Z , 0..9, “ “), and the right and left arrows to move over the name, then press OK to confirm. Use DEL to return to the previous screen. 5.2.1.2 1° Screen METHOD TYPE xxxxxxx ZERO xxxxxxx UNIT xxx Select: Method type (END POINT, KINETIC, FIXED TIME, ELISA). Zero (WATER, REAGENT BLANK, SAMPLE BLANK). Measure unit (mg/dl, mg/dl, g/dl, mg/l, mg/l, g/l, UI/l, UI/ml, mEq/l, mmol/l, mmol/l, %). Selection keys: Use the up & down arrow keys to choose the selection field, and the right and left arrow keys to select the option, then press OK to confirm. Use DEL to return to the previous screen. 5.2.1.3 2° Screen METHOD TEMP xx CALIB xxxxxxx Method temperature (NONE, 25°C, 30°C, 37°C). Calibration mode (FACTOR, STANDARD, CURVE). Selection keys: Use the up & down arrow keys to choose the selection field, and the right and left arrow keys to select the option, then press OK to confirm. Use DEL to return to the previous screen. 3° Screen Different screens will appear according to the calibration mode: a. For Factor Calibration: METHOD FACTOR xxxxxxxxx Select: The numeric value for the factor. The range for the input value is ( 0.00199999 ). Selection Keys: Use the number keys to enter the numeric value, and the right & left arrow keys to move over the number, then press OK to confirm. Use DEL to return to the previous screen . b. For Standard Calibration: METHOD STANDARD ug/dl XXXXX Select: The numeric value for the standard concentration. The range for the concentration value is ( 0.00199999 ). Selection keys: Use the number keys to enter the numeric value, and the right & left arrow keys to move over the number, then press OK to confirm. Use DEL to return to the previous screen . c. For Curve Calibration (multistandard): METHOD N# STD X STD X CONC ug/dl XXX Select: The number of the standard (from 1 up to 6 max). The numeric value for the concentration of each standard. The range for the concentration value is (0.00199999). The range for the standard number ( 1.6 ). Selection Keys: Use the up & down arrow keys to choose the selection field. Use the right & left arrow keys to move over the concentration numeric value and number keys to insert numeric values, then press OK to confirm. Use DEL to return to the previous screen. IMPORTANT NOTE: The standards have to be inserted from the lowest to the highest value In order to display all the concentrations, use the up & down arrow keys to choose the amount of times needed (for example: if 5 has been chosen as the number of the standard and all the standard concentrations have been inserted, use the keys to scroll and check all the standard concentration). 4° Screen METHOD SAMP XXX ul RG 1 XXXX ul RG 2 XXXX ul Select: The sample volume. The reagent 1 volume. The reagent 2 volume. In order to display the rest of the screen selection, respect the following condition: Sample volume + Reagent 1 volume + Reagent 2 volume > =500 µl Selection keys: Use the up & down arrow keys to choose the selection field, the number keys to insert the numeric values and the right & left arrow keys to move over the numbers, then press OK to confirm. Use DEL to return to the previous screen. 5° Screen Select the method wavelength required; the different screens will appear according to the type of the method: For End Point and Elisa methods: METHOD FILTER 1 xxx FILTER 2 xxx Insert: One of the six available wavelengths for the first filter (340, 405, 505, 546, 578, 630 nm). One of the six available wavelengths for the second filter in dichromatic mode (340, 405, 505, 546, 578, 630 nm) or NONE in the monochromatic mode. Selection Keys: Use the up & down arrow keys to choose the selection field, and the right & left arrow keys to select the option, then press OK to confirm. Press DEL to return to the previous screen. b. For Kinetic, Fixed Time: METHOD FILTER 1 xxx Insert: One of the six available wavelengths for the first filter (340, 405, 505, 546, 578, 630 nm). Selection Keys: Use the right & left arrow keys to select the option, then press OK to confirm. Press DEL to return to the previous screen. 6° Screen (only for Kinetic and Fixed time) Select the length of time for measurement: METHOD INC. xxx s. READ xxx s. ABS sec Delay time Insert: The incubation time (INC). The reading time (READ) The delay time is the length of time between the moment at which the sample is placed into the flow cell (or cuvette) and the point when temperature and motion within the sample stabilizes. This delay time is useful to avoid temperature gradient and critic flux movement. In this software it is not programmable and it amounts to 5 seconds. The Incubation time is the period of time that the reaction needs to develop its real ABS gradient. This time is always greater than delay time so the delay is not considered for kinetic and fixed time. In fact the software provides a minimum incubation time of 10 seconds. The Reading time is the period of time the reaction takes to reach its real speed and during this time the instrument achieves one measure per second. ABS sec Delay time Incubation time Reading time The value range for these times is: - Reading time: 1 sec ...300 sec. - Incubation time: 10 sec 600 sec. Selection Keys: Use the up down & arrow keys to choose the selection field, the number keys to insert the numeric values and the right & left arrow keys to move over the numbers, then press OK to confirm. Press DEL to return to the previous screen. 7° Screen N.B: If the following screens are not programmed (i.e. all values are left at zero), the software will not take these values into consideration when checking the results. METHOD NORMAL. MAX xxxxx MIN xxxxx In order to evaluate the result, insert the following values in the appropriate fields on the normality screen. Insert: The maximum value for the concentration (range 0.001 99999). The minimum value for the concentration (range 0.001 99999). Selection Keys: Use the up & down arrow keys to choose the selection field, the number keys to insert the numeric values and the right left arrow keys to move over the numbers, then press OK to confirm. Press DEL to return to the previous screen. 5.2.1.9 8° Screen Select the range for instrument linearity and one of the following screens will be displayed according to the method use: a. For End Point and Elisa methods: METHOD LINEAR. MAX CONC. xxxxx Insert: The maximum value for the concentration (range 0.001 99999) guarantees linearity in the measurement. Selection Keys: Use the number keys to insert the numeric values and the right and left arrow keys to move over numbers, then press OK to confirm. Press DEL to return to the previous screen. b. For Kinetic and Fixed Time: Insert: The maximum valid initial ABS value (range 0.001 99999). Initial maximum ABS value METHOD LINEAR. IN MXABS x.xxx This value is important to evaluate reactions with positive gradient: ABS sec Max value The minimum valid initial ABS value (range 0.001 99999). Initial minimum ABS value METHOD LINEAR. IN MNABS x.xxx This value is important to evaluate reaction with negative gradient: ABS sec Min value The maximum DELTA ABS value (range 0.001 99999), i.e. maximum value for the ABS gradient between the initial and the final values. maximum DELTA ABS value METHOD LINEAR. MAX D ABS x.xxx ABS sec Max delta ABS Selection keys: Use the number keys to insert the numeric values and the right & left arrow keys to move over the numbers, then press OK to confirm. Press DEL to return to the previous screen. 5.2.1.10 Final Screen METHOD SAVE? 1 CONF. 2 QUIT Select Confirm to save the method, the message “ WRITE MEMORY “ will be displayed until the method has been memorized. Select Quit to exit the programming methods without saving. METHOD MEMORY WRITING Selection keys There are two ways to select: 1) Use the up & down arrow keys to move to options over the screen, then press OK to confirm. 2) Use the number keys to make a selection by inserting the row index, for example insert (1) to save the method. 3) Press DEL to return to the previous screen. 5.2.2 Modify method 5.2.2.1 List Screen PROGRAM. 001 GLUCOSE 002 CHOLESTEROL 003 TRIGLYCERIDES Scroll over all the methods in memory and choose the one to be reviewed. Selection keys There are two ways to select: 1) Use the up & down arrow keys to move to different methods stored in memory, then press OK to confirm. 2) Use the number keys to make a selection by digiting the numbers that correspond to the desired method (for example press 024 to review method number 24). 3) Press DEL to return to the previous screen. When the method has been chosen, a message (READ MEMORY) will be displayed and it will last until the method has been loaded from the memory. METHOD MEMORY READING THE OTHER SCREENS ARE THE SAME AS FOR THE “NEW METHOD” PROTOCOL. 5.2.3 Erase method 5.2.3.1 List Screen ERASURE 001 GLUCOSE 002 CHOLESTEROL 003 TRIGLYCERIDES Scroll over all the methods in memory and choose the one to be erased. Selection keys There are two ways to select: 1) Use the direction up down & arrow keys to move to different methods stored in memory, then press OK to confirm. 2) Use the number keys to make a selection by digiting the numbers that correspond to the desired method (for example press 024 to review method number 24). 3) Press DEL to return to the previous screen. When the method has been chosen, a message (READ MEMORY) will be displayed and it will last until the method has been loaded from the memory. METHOD MEMORY READING 5.2.3.2 Confirm Screen METHOD ERASE ? 1 CONFIRM 2 QUIT Confirming or quitting the erasing method. Selection keys There are two ways to select: 1) Use the up and down arrow keys and move to options over the screen, then press OK to confirm. 2) Use the number keys to make a selection by inserting the row index, for example insert (1) to erase the method. 3) Press DEL to return to the INTRO SCREEN. N.B. In order to avoid errors in method running (during a set series of experiments), it is important not to modify the CALIBRATION and the method TYPE once the series of experiments have begun. 5.3 Working on methods This section contains information regarding the execution of methods saved in memory. IMPORTANT NOTE: If in the daily session there are some analyses with method temperature at 25°C, it is recommended to perform these before the others, in order to guarantee best possible operation of the instrument (the instrument will keep on running even if this is not done, but the precaution will ensure a perfect result). Do not use corrosive reagents because they may damage the metal flow cell. List Screen: EXEC. 001 GLUCOSE 002 CHOLESTEROL 003 TRIGLYCERIDES Scroll over all the methods in the memory and choose the one to run. Selection keys There are two ways to select: 1) Use the up & down arrow keys to move to different methods over the screen, then press OK to confirm. 2) Use the number keys to make a selection by digiting the numbers that correspond to the desired method (for example press 024 to review method number 24). 3) Press DEL to return to the MAIN SCREEN. After a method has been chosen, a message (READ MEMORY) will be displayed until the method has been loaded from the memory. METHOD MEMORY READING Print Screen: METHOD PRINT ? 1 YES 2 NO To decide whether or not to print the method (please refer to PRINT MENU for information about printing format) press: 1° option: to print the method. 2° option: not to print the method. There are two ways to select: 1) Use the up & down arrow keys to move to different options over the screen, then press OK to confirm. 2) Use the number keys to make a selection by inserting the row index, for example insert (1) to print the method. 3) Press DEL to return to the MAIN SCREEN. 5.3.1 Zero-setting The zero protocol is performed each time the zero type is required: When the water zero or the reagent blank zero is chosen, the zero protocol is performed only once, at the beginning of the running method. When the sample blank zero is chosen, the zero protocol is performed at the beginning of each measurement (standard or sample measurement) of the relevant running session. 5.3.1.1 Zero Acquire Screen This screen is displayed when no zero absorbance value is stored. METHOD ABS BLANK (or water) DEL. 1) Set up (according to the zero type) the water/blank test tube and fill the flow-cell by pressing the PUSH button. 2) Wait for a fixed delay time (5 sec.) for the environment to stabilize. 3) Calculated ABS blank value will be displayed (0.000) This screen runs only one measure when a double filter method (dichromatic) is used. Selection keys: Press the PUSH button to fill the flow cell with the sample. Press DEL to return to the MAIN SCREEN. Zero Confirm Screen METHOD ZERO OK? 1 YES 2 NO ABS xxxxxxx This screen is displayed when a previous zero absorbance value has been stored. This value is displayed for a few seconds and it may be accepted or refused. To decide whether or not to accept the value press: 1° option: to accept the value. 2° option: not to accept the value. There are two ways to select: 1) Use the up & down arrow keys to move on different options over the screen, then press OK to confirm. 2) Use the number keys to make a selection by inserting the column index, for example insert (1) to accept the value. 3) Press DEL to return to the MAIN SCREEN. 5.3.2 Sample blank zero Setting When sample blank zero is selected, the zero must be performed for each test (both sample and standard). When zero is selected, the screen will appear in the following sequence: If a curve factor value is stored in the memory, a CURRENT STANDARD CONFIRM SCREEN will appear after the PRINT SCREEN. Before pressing the PUSH button, after every standard or sample measurement, it is necessary to fill the flow cell with zero solution again, after this operation it is possible to proceed to a new standard or sample measurement. N.B: In NEW STANDARD MEASUREMENT SCREEN (see below) after selecting the standard that has to be repeated, prepare and position the zero solution under inlet pipe then press PUSH button, the zero will be achieved. Then position the standard which is to be read under the inlet pipe and press the PUSH button. 5.3.3 Production of Standards The standard protocol is not performed with the Factor calibration so, in this case, after the zero setting, the programme goes directly to the sample measurement (please refer to the section concerning sample measuring). The standard measurement is performed any time the standard calibration type requires: When the standard calibration is selected, perform the standard measurement (see the STANDARD MEASUREMENT SCREEN). When the curve calibration is selected, perform for each standard a measurement (see STANDARD MEASUREMENT SCREEN) for the number of times equal to the number of repetitions programmed in the method. IMPORTANT NOTE Every time that you change the following parameters: Method type. Zero type. Calibration type. Filters. Volumes (sample or regent). Incubation and reading time. The stored standards are erased. 5.3.3.1 Current standard confirm screen If the current method has been performed at least once, the old standard results will be retained in the memory and must be reviewed, confirmed, or modified. If instead the current method has not been previously performed, this screen not appear and the STANDARD MEASUREMENT SCREEN will automatically be displayed. The program will display the following screen when the standard calibration has been selected. You can see the current factor. This value may be accepted or refused: METHOD FACT. OK? xxxxxxxxxx 1 YES 2 NO To decide whether or not to accept the value press: 1° option: to accept the value. 2° option: not to accept the value. There are two ways to select: 1) Use the right & left arrow keys to move to different options over the screen, then press OK to confirm. 2) Use the number keys to make a selection by inserting the column index, for example insert (1) to accept the value. 3) Press DEL to return to the MAIN SCREEN. If you choose to accept the value, a SAMPLE MEASUREMENT SCREEN appears (see below). If you choose to not accept the value, the following screen will be displayed: METHOD 1 NEW 2 OLD Select OLD for recovering the last factor memorized. Select NEW for making a new factor: A STANDARD MEASUREMENT SCREEN will appear (see below). N.B. The first time that the programme runs the old factor is the current factor. There are two ways to select: 1) Use the up & down arrow keys to move to different options over the screen, then press OK to confirm. 2) Use the number keys to make a selection by inserting the row index, for example insert (1) to select NEW option. 3) Press DEL to return to the previous screen. The following screen is displayed when the curve calibration has been selected. CURRENT STANDARD CONFIRM SCREEN METHOD ABS OK? 1 YES 2 NO ug/dl Selection keys: 1) Use the up & down arrow keys to move to different options over the screen, then press OK to confirm. 2) Use the number keys to make a selection by inserting the row index, for example insert (1) to accept the standard. 3) Use the question mark key ( ? ) to print the calibration curve. 4) Press DEL to return to the MAIN SCREEN. CALIBRATION CURVE ABS 1.000 0 ¨ x 10 ug/dl ug/dl ABS x 1 1.000 ¨ 2 .0.000 N.B. During the graph printing mode, all other operations will be inhibited for several seconds. If the current standard is acceptable select YES and the SAMPLE MEASUREMENT SCREEN will appear (see below). If the current standard is not acceptable select NO and a STANDARD MEASUREMENT SCREEN will appear (see below) if it is the first time which the method runs, otherwise the following screen will appear: METHOD 1 NEW 2 OLD N.B. The first time that the programme runs the old standard session is the current standard session. If the current standard session is not acceptable, you have more possibilities: To perform a complete new standard measurement session select NEW and press OK, so a STANDARD MEASUREMENT SCREEN appears. To recover completely an old standard measurement session (already present in the instrument memory) select OLD and press OK, so a CURRENT STANDARD CONFIRM SCREEN appears. To replace one or more standards from an old multi-calibration measurement session (already present in the instrument memory) select NEW and use the right and left arrows keys to choose the standard. The following screen appears: METHOD 1 REP. N# X 2 OLD Then press OK to confirm. A STANDARD MEASUREMENT SCREEN will be displayed. If the new measurement is unsatisfactory, it is possible to recover the previous standard. Select OLD and use the right and left arrows keys to choose the standard. The following screen appears: METHOD 1 NEW 2 REC. N# X Then press OK to confirm. A CURRENT STANDARD CONFIRM SCREEN will be displayed. Press OK to confirm new or old standard measurement values in instrument memory and proceeded to sample measuring (see 5.4.4 ) 5.3.3.2 Standard measurement screen PLEASE PAY ATTENTION: For Kinetic and Fixed Time methods the ABS symbol has to be considered as a delta absorbance value. Current standard order number ABS value Flow-cell temperature Time evolution. Reaction graph STD X ABS XX.X °C To run the program, set up the standard test-tube then press the PUSH button. The instrument will fill the flow cell, take a measurement, and then expel the standard. During the incubation time for Kinetic and Fixed Time methods or the delay time for End Point and Elisa methods the program will display the following screen: STD X ABS xx XX.X °C This screen displayed: Time evolution. The current standard order number, if the standards are more than one. The current working temperature of the flow cell. For Elisa and End Point methods, after the delay time, the absorbance value (ABS) and its concentration value are displayed on the screen and printed. During the reading time for Kinetic and Fixed Time methods the screen displayed: The delta absorbance evolution and the initial and final absorbance values. The current standard order number, if the standards are more than one. The reading time evolution. The current working temperature of the flow cell. After the first ten seconds of the reading time, a real time graphic dynamics of the measurement is shown on screen. The graphic point could to be dispersed because the real trend is displayed. The screen is the following for reactions with positive gradient: x.xxx x.xxx STD X ABS xxxx xx XX.X °C The screen is the following for reactions with negative gradient: x.xxx x.xxx STD X ABS xxxx xx XX.X °C After a few seconds, the following data will be printed: The result of the standard measurement. The concentration value. The dynamics curve. The dynamics curve is the best fit curve calculated on four point, for a good estimate of reaction speed on quarters of reading time. Instead, the measure is made on the real trend. DYNAMICS ABS 0.963 0.886 x ¨ ˆ x 30s. 60s ABS x 15 s .0.005 ¨ 30 s 0.009 ˆ 45 s ....0.013 x 60 s .0.015 N.B. During the graph printing mode, all other operations will be inhibited for several seconds. If you are performing a CURVE calibration, to proceed to the next standard (a new STANDARD MEASUREMENT SCREEN session will be displayed) press the PUSH button and keep on working this way until the last standard has been performed. When the last measurement has been taken, a new CURRENT STANDARD CONFIRM SCREEN will appear. Selection keys: Press the PUSH button to fill the flow-cell with the standard. Press DEL to return to the MAIN SCREEN. 5.3.4 Sample measuring It is now possible to measure the samples. 5.3.4.1 Sample measurement screen PLEASE PAY ATTENTION: For Kinetic and Fixed Time methods the ABS symbol has to be considered as a delta absorbance value. Time evolution. The current sample order number. The current working temperature of the flow cell. SAMP X ABS XX.X °C To run the program, set up the test-tube with the sample and press the PUSH button. The instrument will fill the flow cell, take a measurement, and then expel the sample. During the incubation time for Kinetic and Fixed Time methods or the delay time for End Point and Elisa methods the programme will display the following screen: SAMP X ABS xx XX.X °C This screen displayed: Time evolution. The current sample order number. The current working temperature of the flow cell. For Elisa and End Point methods, after the delay time, the result of the ABS standard measurement and the concentration value are displayed. The result of the sample concentration has a supervisor control. If a question mark is displayed after the number, the measurement value is out of controls. ug/dl SAMP X ABS x.xxx x.xx xx XX.X °C During the reading time for Kinetic and Fixed Time methods the screen displayed: -The current sample order number. -The delta absorbance evolution and the initial and final absorbance values. -The reading time evolution. -The current working temperature of the flow cell. -After the first ten seconds of the reading time, a real time graphic dynamics of the measurement is shown on screen. The graphic point could to be dispersed because the real trend-is-displayed. -The result of the sample concentration at the end of the measurement, with a supervisor control. If a question mark is displayed the concentration value is out of controls. The screen is the following: x.xxx x.xxx ug/dl SAMP X ABS xxxx x.xx xx XX.X °C After a few seconds, the following data will be printed (this for all the methods): METHOD NAME TYPE: Type of reading for the current method. DATE and OPERATOR CODE. NO: Current sample number (or current standard). ABS: Absorbance value for sample (or standard absorbance). RESULT: The concentration value (or standard value). H or L: The out of normality range. OL: The out of linearity range. 015 GLUC ________________________ TYPE END POINT DATE 10/06/04 OPERATOR CODE 01 ________________________ NO. ABS RESULT STD 0.340 100 1. 0.450 132 2. 0.412 121 3. 0.840 247 H 4. 1.600 500 H-OL N.B. During the graph printing mode, all other operations will be inhibited for several seconds. For Kinetic and Fixed Time types if question mark key (?) is pressed dynamics curve will be printed: DYNAMICS ABS 0.963 0.886 x ¨ ˆ x 30s. 60s ABS x 15 s .0.005 ¨ 30 s .0.009 ˆ 45 s ....0.013 x 60 s .0.015 The dynamics curve is the best fit curve calculated on four points for a good estimate of reaction speed on quarters of reading time. Instead, the measure is made on the real trend. N.B. During the graph printing mode, all other operations will be inhibited for several seconds. To pass to the next sample (a new SAMPLE MEASUREMENT SCREEN session will be displayed), press the PUSH button and keep on working this way. If the programme finds zero value for all the controls, it will not take them into consideration. N.B: In particularly cold environments the incubator might incur a loss of temperature after the Kinetic analyses, so it is suggested to exit the running method and to wait for few seconds in order to allow the incubator to hit up again. Selection keys: 1)Press the PUSH button to fill the flow cell with sample. 2)Press question mark key ( ? ) to print the dynamics for KINETIC, FIXED TIME types only (with other types the question mark key ( ? ) worn out of range value ). 3) Press DEL to return to the MAIN SCREEN. ABS Mode ABS mode is a service function, useful to test the ABS of sample with a programmable wavelength. To use this function first you have to set the instrument to zero with a reference (for example water or reactive). 5.4.1 ABS Mode Selection Screen ABS FIL. xxx VOL. xxx The filter wave length ( 340 , 405 , 505 , 546 , 578 , 630 ). The sample volume ( minimum value 500 ul ). Selection keys: 1) Use the up & down arrow keys to choose the selection field and the right and left arrow keys to select the option, then press OK to confirm. 2) Use the number keys to insert the numeric values and the right & left arrow to move over numbers, then press OK to confirm. 3) Press DEL to return to the MAIN SCREEN. 5.4.2 ABS Mode Measurement Screen ABS XXX ZERO When the program arrives to this screen, the instrument will set up the optical filter (showing the current wavelength). Set the test tube of water (or other substances) in the inlet pipe and press the PUSH button. After a few seconds, continuous evolution of the ABS value will appear on the display. During the screening the following options must be chosen: Press 0 key to have zero on the current ABS value (water or other substances). You will choose this option when you will set to zero the instrument. Prepare a new sample (reagents, potassium-dichromate or others) and press the PUSH button to start a new ABS measurement session. At the screen exit, the instrument expels the volume from flow cell via the outlet waste. Selection keys: Press the PUSH Button to fill the flow cell with the sample. Press 0 key to choose the zero value. Press DEL to return to the ABS MODE SELECTION SCREEN. Washing After each session of measurements, it is recommended that the flow cell be washed with distilled water. Washing is possible every time, within the EXE Mode or within the ABS Mode. For washing the flow cell press the point key ( . ) so the pump will drain away the water at high speed into the flow cell. After the washing, it is necessary to repeat the operation without water so that the water remaining inside the instrument can be expelled completely. It is very important to not alter the following measure. 5.6 Print Menu Methods can be printed without running them. Printing Option Screen PRT 001 GLUCOSE 002 GPT 003 UREA UV Press DEL to return to the MAIN SCREEN. Press the arrow right key to print the list of methods stored in memory. The format is the following: METHOD LIST 001 GLUCOSE 002 GPT 003 UREA UV During the printing action the programme will show a message with the status of the instrument: PRT To print a single method, scroll through all the methods in memory and choose the one to be printed. After the method has been chosen, the message READ MEMORY will be displayed which will last until the method has been loaded from the memory. METHOD READ MEMORY The printing will then begin and will appear in the following format: 003 UREA UV TYPE: FIXED TIME ZERO: WATER UNIT: mg/dl TEMP : 37 STD N. 1 STD.1, CONC .50.0 mg/dl CALIB.: STD FACTOR : 1746 SAMPLE V. 5ul REAG. 1 V: 500ul REAG. 2 V: 0 ul READING TIME. : 60 s. INCUB. TIME. : 30 s. FILTER : 340 NORMALITY MAX 50.00 mg/dl MIN 10.00 mg/dl During the printing action the programme will show a message with the status of the instrument: PRT N.B. During printing action, all other operation will be inhibited for some seconds, according to the length of the text being printed. The controls for linearity and normality are only printed if relevant values are meaningful (not zero). Selection keys There are two ways to select: 1) Use the up & down arrow keys to move to different options over the screen and methods stored in memory, then press OK to confirm. 2) Use the number keys to make a selection by digiting the numbers that correspond to the desired method (for example press 024 to review method number 24). 3) Press DEL to return to the MAIN SCREEN. Using Manual Cuvette If there are problems using the flow cell, it is also possible to use the manual cuvette, see also paragraph 4.9 page 9: Cuvette position Pump Take out the flow cell and its adapter Insert the ADAPTOR for manual cuvette into the cuvette position. Make sure that it is pushed firmly inside. If using the cuvette ADAPTOR, place into the manual cuvette at least 500 µL of sample. Then insert the manual cuvette on the cuvette position over the adapter, cover with black cylinder then press the PUSH button. Make sure to position the manual cuvette correctly: Cuvette position Manual cuvette position Manual cuvette Screw Screww Close the measurement block door before pressing the PUSH button. 5. MAINTENANCE Cleaning procedures: Press point key (.) to activate the peristaltic pump for flow-cell washing. The peristaltic pump will aspire for several seconds in order to aspirate distilled water or cleaning solutions. This feature is activable in each instrument menu (also during sample readings) 6.1.1 First installation Perform at least 10 complete washing cycles by using the Washing procedure. Every washing cycle lasts approx. 1 minute and stops automatically. 6.1.2 Everyday cleaning At the end of each working session, it is recommended to rinse the instrument with detergent solution or alcohol via the inlet pipe. Place a cup of detergent solution under the inlet pipe and carry-out the Washing procedure. 6.1.3 Special cleaning Every month perform at least 2 complete washing cycles with sodium hypochlorite (bleach) by using the Washing procedure. N.B.: Do not use corrosive detergents to wash the instrument. 6.2 Further advice Further advice for correct use and maintenance of the instrument: If the instrument is not being used for a long period of time, empty the hydraulic circuit and disconnect the tubes. When the instrument is in use, keep the two little doors of the instrument (measurement block and printer doors) firmly closed. Clean the external surface of the instrument every month with a non abrasive detergent. Clean the surface near the two fans every month in order to take off dangerous dust. 6.3 Changing lamp procedure When the lamp is damaged, it is necessary to replace it with a new one by following this procedure: Open case of the instrument, remove the four screws under the equipment and the three screws on the back. Remove the two screws at right and left side of flow cell site, and move aside the pump support (with pump rotor). Move aside the little tongue that fixes the lamp, by unscrewing the screw. Take out old lamp and insert the new one carefully, bringing it up to the bottom of the site (pay attention to not touch lamp glass with bare finger). Replace the little tongue, and check that the top of the lamp is inside the little hole. Set up again pump support and equipment case.